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Project TitleBacteria Modified to Secrete Biologically Active Protein for Large-Scale Production
Track Code5477
Short Description

Researchers have discovered E. coli mutations that substantially increase the amount of biologically active, recombinant protein secreted from cells.

 

Abstract

The mutations disrupt genes in a YebF-mediated protein secretion pathway. Bacteria modified to contain these mutations are useful for the production of secreted proteins. They can be used to produce proteins that might otherwise not be expressed due to toxicity or folding errors. They also can be used to produce secreted complexes of enzymes such as cellulases and xylanases for the manufacture of cellulosic biofuels.

 

Potential Applications:

  • Manufacturing proteins for bioenergy production, therapeutic biologics and research tools
  • Rapid, high-throughpout production of proteins on a commercial scale.

 

Additional Information:

  • Issued Patents in the U.S. 9,410,157 and 9,574,197

  • Patent filed in Canada CA2747462

  •  Haitjema C. H. (2014). Universal Genetic Assay for Engineering Extracellular Protein Expression. ACS Synth. Biol., 3(2):74–82 DOI: 10.1021/sb400142b.
 
Tagslife science, proteins, bacterial expression
 
Posted DateSep 27, 2012 2:16 PM

Researchers

Matthew DeLisa

Miguel A. Dominguez

Charles H. Haitjema

Sydnor T. Withers III

Licensing Contact

Phillip Owh
po62@cornell.edu
1-607-254-4508

Files

File Name Description
Technology Brief D5477 FlAsH-tetracysteine-based Assay Download